List of protocols used in the lab at URI for lab-a-thon in March 2024!

Protocols used this week at URI for lab processing

• Zymo MiniPrep Plus RNA and DNA Extractions
  • Jill’s Zymo Quick MiniPrep plus RNA/DNA extraction protocol and modified protocol
  • Jill’s post of our proceedure with modifications
  • Zymo’s published extraction protocol
• Gel electrophoresis
  • Lab Gel protocol
• Tapestation quantification
  • Maggie’s Tapestation protocol
• Qubit quantification
  • Lab Qubit protocol
• ITS2 PCR amplification and preparation for next gen sequencing at RI-INBRE
  • Ariana’s updated ITS2 amplification and prep protocol
  • Emma’s ITS2 amplification and prep protocol
  • Our proceedure with modifications that we ran this week modified in our protocol
• Pocillopora POC Histone RFLP
  • Hollie’s Pocillopora species ID protocol and Zoe’s mtORF and POC Histone protocol
  • Our proceedure with modifications that we ran this week modified in our protocol
• Porites POR H2 PCR and preparation for Sanger sequencing
  • Zoe’s Porites H2 protocol
  • Our proceedure with modifications that we ran this week modified in our protocol
• Zymo Switch 3’ library prep
  • Jill’s Zymo Switch 3’ library prep protocol and post of our work this week
  • Zymo’s published Switch 3’ library prep protocol