This post details preparations for outplanting 10K Seed at Baywater shellfish.

Overview

In this project, we exposed oyster seed (C. gigas) at the Point Whitney Shellfish Hatchery to 5 different stress hardening treatments:

• Control (no treatment)
• Immune
• Temperature (35°C)
• Fresh water
• Fresh water at high temperature (35°C)

We conducted the hardening treatments at Point Whitney over the last few months. Now, we are preparing these oysters for outplanting to monitor field performance at Baywater Shellfish in the Hood Canal.

See my previous notebook posts here for more details on this project.

This post shows the results of survival curve analysis from metabolic rate incubations using resazurin. For more information on these incubations, visit Colby’s notebook. I’ll make a post with a full resazurin protocol soon.

Preparing outplants

Today we prepared 10K seed for outplanting at Baywater Shellfish, which will occur at night on Oct 30th. We counted out three groups of 150 seed from each of the 10 seed bags. This resulted in n=3 groups from each bag. Bags are duplicates of treatments, so we have a total of n=6 groups per treatment.

Here is the metadata.

purple.tag	source.bag	treatment	number.oysters
1	75	FW 35C	150
2	75	FW 35C	150
3	75	FW 35C	150
4	76	FW 35C	150
5	76	FW 35C	150
6	76	FW 35C	150
7	47	35C	150
8	47	35C	150
9	47	35C	150
10	30	35C	150
11	30	35C	150
12	30	35C	150
13	49	Immune	150
14	49	Immune	150
15	49	Immune	150
16	50	Immune	150
17	50	Immune	150
18	50	Immune	150
19	66	FW	150
20	66	FW	150
21	66	FW	150
22	56	FW	150
23	56	FW	150
24	56	FW	150
25	37	Control	150
26	37	Control	150
27	37	Control	150
28	36	Control	150
29	36	Control	150
30	36	Control	150

We took an image for size of one group of 150 seed per bag for initial size measurements.

I also launched three temperature loggers to deploy at Baywater.

• 22023411: new logger
• 22023406: previously with broodstock, downloaded and relaunched
• 22023410: previously with lifestage carryover, downloaded and relaunched

All loggers were launched to log data every 15 minutes starting at 10:00pm on 10/30/2024.

Finally, I took a subsample of length, width, and depth measurements (recorded in notebook images below) in order to model depth as a function of length and width. We can use this to then infer depth from length and width in size images. I need to enter these data and analyze.

LCO size measurements

I took final size measurements of all LCO oysters and images of spat. The measurements are on GitHub here as notebook images. I need to enter these data and take size measurements from spat images.

All oysters and shells were disposed of in the lagoon and are now cleaned out.

Inventory

I consolidated all remaining bags of oysters (PH families, Effort C bags) in one 3-holer at Point Whitney. These will remain running over the winter.

Sampling juveniles for Mac

I took ~20 juveniles of the 2024 USDA families in a cooler for Mac.

Notebook images