This post details activities at Point Whitney from 10 July 2024.

Overview

Today I ran the continuing 10K seed stressors and weekly broodstock stressor at Point Whitney.

Cleaning tanks

I didn’t see any larvae in our HUDLs, so I drained and cleaned them and left the tank empty. I kept the water lines running to allow the algae tank to drain. I turned off all heaters and rinsed all tanks.

Probe measurements

Measurements are on GitHub here.

Stressor measurements are on GitHub here.

Broodstock weekly stressors

Broodstock from bags 99 and 100 were added to a bucket with fresh water from 10:20-10:50.

Stressors for new/10K seed

Today we did stressors for Kathleen’s experiment by increasing temperature in the right tank to 35°C with a few inches of water. We had some electrical overload problems, so we ultimately did this using 1 800W heater and 2 300-500W heaters. All heaters were add to the 35°C tank to get it up to temperature. Then, one heater was put back in the larval tank set to 28°C and one went into a bucket of fresh water to heat to 35°C.

I did the following stressors:

1. New PW seed: Freshwater for 1 hour in a bucket. Done from 10:30-11:30.
2. New PW seed: 35°C seawater for 1 hour in the right tank. Done from 11:00-12:00.
3. New PW seed: 35°C freshwater for 1 hour in a heated bucket. Done from 10:30-11:30.

We will continue the MWF stressors through this week and I will take all effort B/E/D oysters to UW along with a subsample of the 10K seed next week for testing and sampling.

LCO check in

I didn’t see any mortality in LCO oysters. The bags were really dirty in the tank, so I moved them into a 3-holer well in the same tank.

Notebook images

To do list for 7/12/2024

1. Open broodstock to look for gametes
2. 10K seed stress: PolyIC, fresh, 35C seawater, 35C fresh water stressors