This post details activities at Point Whitney from 28 June 2024.

Overview

Today Kathleen and I were at Point Whitney to run a round of stressors on Kathleen’s triploid/diploid experiment and conduct stressors on broodstock and new seed.

Checking on larvae

I checked on the larvae today and dropped all HUDLs onto a 40um screen. I looked for larvae and saw very few, we likely lost most of the larvae.

I cleaned all HUDLs and put larvae back in. I also increased water flow to all HUDLs to be equal and turned up the water to the header to meet the demand.

Fluorometry measurments in HUDLs were 1, 2, and 2 when I came in at 11:00. This is likely because the food header was empty. I filled it and at 15:00 the levels were at 24, 32, and 30!

Probe measurements

Weekly tank measurements:

tank	date	time	temp.C	pH.nbs	sal.psu	initials	notes
HUDL	6/28/24	11:15	26.1	8.13	30.37	ash	larvae
HUDL	6/28/24	11:15	26.2	8.13	30.38	ash	larvae
HUDL	6/28/24	11:15	26.3	8.14	30.36	ash	larvae
upweller	6/28/24	11:15	19.4	7.56	28.84	ash	effort C
upweller	6/28/24	11:15	19.6	7.47	29.07	ash	broodstock
upweller	6/28/24	11:15	19	7.54	28.93	ash	efforts BED
outdoor_tray	6/28/24	11:15	15.2	7.62	29.2	ash	left stack
outdoor_tray	6/28/24	11:15	16.7	7.59	29.29	ash	right stack
dock	6/28/24	11:15	18.9	7.62	29.29	ash	seed

Measurements are on GitHub here.

Stressor treatments (see below for more info):

treatment	date	time	temp.C	pH.nbs	sal.psu	initials	notes
fresh	6/28/24	12:00	16.8	7.59	0.374	ash	broodstock
fresh	6/28/24	12:00	14.2	8.07	0.522	ash	seed
temperature 35C	6/28/24	12:20	33.8	7.64	31.03	ash	right tank
temperature 35C	6/28/24	12:50	34.1	7.67	30.85	ash	right tank
temperature 35C	6/28/24	13:20	34.3	7.69	31.41	ash	right tank
temperature 35C	6/28/24	15:00	35.1	7.75	31.32	ash	right tank
fresh temperature 25C	6/28/24	01:07	32.7	8.29	0.164	ash	bucket
fresh temperature 25C	6/28/24	01:20	35	8.24	0.419	ash	bucket

Stressor measurements are on GitHub here.

Broodstock weekly stressors

Broodstock from bags 99 and 100 were added to a bucket with fresh water from 11:43-12:13.

Kathleen’s stressors and stressors for new seed

Today we did stressors for Kathleen’s experiment by increasing temperature in the right tank to 35°C with a few inches of water. We had some electrical overload problems, so we ultimately did this using 1 800W heater and 2 300-500W heaters. All heaters were add to the 35°C tank to get it up to temperature. Then, one heater was put back in the larval tank set to 28°C and one went into a bucket of fresh water to heat to 35°C.

We did the following stressors:

1. Kathleen: Diploid and triploid groups at 35°C in seawater for 4 hours (bags 60 and 65). Done from 12:12-16:00.
2. New PW seed: Freshwater for 1 hour in a bucket. Done from 11:45-12:46.
3. New PW seed: 35°C seawater for 1 hour in the right tank. Done from 12:33-13:33.
4. New PW seed: 35°C freshwater for 1 hour in a heated bucket. Done from 13:07-14:07.

There are 10 total bags of seed with ~1K seed each sourced form Point Whitney commercial hatchery. Here are the bags inventoried with their treatments. We did the temperature and fresh treatments today and will do immune treatments once we decide on methods. Steven has a post about these seed here.

Kathleen’s bag inventory and new seed inventory:

cattle_tag	effort	treatment_type	treatment	family	source	tag_description	location
45	KD	2 week temperature Triploid	Control	Bird	Pacific Hybreed	Triploid Control	Outdoor upweller; Point Whitney
60	KD	2 week temperature Triploid	Treated	Bird	Pacific Hybreed	Triploid Treated	Outdoor upweller; Point Whitney
64	KD	2 week temperature Diploid	Control	13	Pacific Hybreed	Diploid Control	Outdoor upweller; Point Whitney
65	KD	2 week temperature Diploid	Treated	13	Pacific Hybreed	Diploid Treatment	Outdoor upweller; Point Whitney
66	PW Bulk Seed	2 week stressors	Treated FW	PW Seed	PW	UW-SR26	Dock lagoon; Point Whitney
56	PW Bulk Seed	2 week stressors	Treated FW	PW Seed	PW	UW-SR6	Dock lagoon; Point Whitney
36	PW Bulk Seed	2 week stressors	Control	PW Seed	PW	UW-SR27	Dock lagoon; Point Whitney
37	PW Bulk Seed	2 week stressors	Control	PW Seed	PW	UW-SR30	Dock lagoon; Point Whitney
50	PW Bulk Seed	2 week stressors	Immune	PW Seed	PW	UW-SR23	Dock lagoon; Point Whitney
49	PW Bulk Seed	2 week stressors	Immune	PW Seed	PW	UW-SR30	Dock lagoon; Point Whitney
47	PW Bulk Seed	2 week stressors	Treated 35C	PW Seed	PW	UW-SR14	Dock lagoon; Point Whitney
30	PW Bulk Seed	2 week stressors	Treated 35C	PW Seed	PW	UW-SR18	Dock lagoon; Point Whitney
75	PW Bulk Seed	2 week stressors	Treated FW35C	PW Seed	PW	UW-SR5	Dock lagoon; Point Whitney
76	PW Bulk Seed	2 week stressors	Treated FW35C	PW Seed	PW	UW-SR21	Dock lagoon; Point Whitney

Here is the schedule of the stressor treatments with cattle tag numbers for each.

F June 28	M July 1	W July 3	F July 5	M July 8
AH/KD	KD	KD	KD	KD
4 hour 35 ploidy (60, 65)	4 hour 35 ploidy (60, 65)	4 hour 35 ploidy (60, 65)	4 hour 35 ploidy (60, 65)	4 hour 35 ploidy (60, 65)
1 hour 35 seed (47, 30)	1 hour 35 seed (47, 30)	1 hour 35 seed (47, 30)	1 hour 35 seed (47, 30)	1 hour 35 seed (47, 30)
1 hour FW seed (66, 56)	1 hour FW seed (66, 56)	1 hour FW seed (66, 56)	1 hour FW seed (66, 56)	1 hour FW seed (66, 56)
1 hour FW 35 (75, 76)	1 hour FW 35 (75, 76)	1 hour FW 35 (75, 76)	1 hour FW 35 (75, 76)	1 hour FW 35 (75, 76)
Check larval survival and drop HUDLS	Tank probe measurements	Tank probe measurements	30 min FW Broodstock stress (99, 100)	Tank probe measurements
30 min FW Broodstock stress (99, 100)			Tank probe measurements
Tank probe measurements

This updated tag inventory is on GitHub here.

I also launched a logger SN 22023399 to log temperature every 15 minutes starting on 6/28/2024 at 14:00. This was zip tied onto one of the dock bags for the new seed.

During stressors, control oysters were left at their respective tank locations. Stressed oysters were returned to locations after stressor.

Notebook images