This post details activities at Point Whitney from 24 June 2024 to prepare Goose Point outplants.

Overview

Today I went to Point Whitney to check larvae, take probe measurements, and prepare Goose Point outplants.

Checking on larvae

I checked on the larvae today that Steven and Carrie put in the HUDLs last week. I poured the contents of one HUDL onto a 40um screen and rinsed the larvae into a small volume.

I then viewed the larvae under the scope.

They are alive! There is a range of sizes and they have some algae in their gut. They could definitely be feeding more. Fluorescence measurements were between 2 and 4 in the HUDLs today. I increased the pump rate from 25 to 40. Steven will check the readings tomorrow to see if it has increased.

I estimated about 40-50K larvae in the one HUDL that I dropped. Larvae were returned to the HUDL. Temperatures look good.

I added EDTA at 1g/1000L concentration at 11:30. I mixed 0.2g EDTA with some hot fresh water, mixed, and added to our 55 gallon header (208 L).

Probe measurements

Weekly tank measurements:

tank	date	time	temp.C	pH.nbs	sal.psu	initials	notes
HUDL	6/24/24	10:45	27.6	8.1	30.62	ash	larvae
HUDL	6/24/24	10:45	27.9	8.07	30.55	ash	larvae
HUDL	6/24/24	10:45	27.6	8.09	30.4	ash	larvae
upweller	6/24/24	10:45	18.6	7.63	29.17	ash	effort C
upweller	6/24/24	10:45	18.5	7.65	29.13	ash	broodstock
upweller	6/24/24	10:45	18.9	7.63	29.12	ash	efforts BED
outdoor_tray	6/24/24	10:45	16.8	7.61	29.2	ash	left stack
outdoor_tray	6/24/24	10:45	15.1	7.64	29.42	ash	right stack

Measurements are on GitHub here.

Stressor treatments (see broodstock info below):

treatment	date	time	temp.C	pH.nbs	sal.psu	initials
fresh	6/24/24	10:45	16.9	8.39	0.228	ash
control	6/24/24	10:45	16.5	8.09	30.12	ash
fresh	6/24/24	10:45	15.7	8.51	0.198	ash

Stressor measurements are on GitHub here.

Broodstock stressors

I stressed the treated broodstock oysters that I started last week. I added them to fresh water (see probe measurements above) from 10:30-11:00. There was no mortality from stressing them last week. The control oysters from the same families were kept indoors at ambient conditions during the exposure. Returned to upwellers.

An updated tag inventory is on GitHub here.

Prepping for Goose Point outplants

Today I took all oysters out of their seed bags for Effort E seed for outplanting at Goose Point. I was able to get a maximum of 100 seed per bag for a total of 14 bags. I then set out each set of 100 with their bag tag and took a photo with a scale for size measurements. I was able to get n=3 bags for all treatments, except for the fresh water control, which had more oysters and I was able to get n=6 bags.

The oysters were then kept in a cooler with ice for transport to Goose Point tomorrow! Field tags will be added to this table tomorrow when we outplant and attach tags. I’ll also take the temperature loggers that I started last week.

bag	effort	treatment	source_cattle_tag	field_cattle_tag	number_oysters	photo_taken
UW-SR33	Effort E	Weekly Temperature Control	1		100	yes
UW-SR34	Effort E	Weekly Temperature Control	1		100	yes
UW-SR35	Effort E	Weekly Temperature Control	1		100	yes
UW-SR36	Effort E	Weekly Temperature Treated	20		100	yes
UW-SR37	Effort E	Weekly Temperature Treated	20		100	yes
UW-SR38	Effort E	Weekly Temperature Treated	20		100	yes
UW-SR39	Effort E	Weekly Fresh Water Control	24		100	yes
UW-SR40	Effort E	Weekly Fresh Water Control	24		100	yes
UW-SR41	Effort E	Weekly Fresh Water Control	24		100	yes
UW-SR42	Effort E	Weekly Fresh Water Control	24		100	yes
UW-SR43	Effort E	Weekly Fresh Water Control	24		100	yes
UW-SR44	Effort E	Weekly Fresh Water Treated	23		100	yes
UW-SR45	Effort E	Weekly Fresh Water Treated	23		100	yes
UW-SR46	Effort E	Weekly Fresh Water Treated	23		100	yes

Here are example images of what I took for size measurements for all oysters in each bag:

Data for Goose Point outplanting is on GitHub here.

Images for sizes for all Goose Point outplants is on GitHub here.

Notebook images