This post details RNA extractions for the 2023 Moorea Symbiotic Exchange project, which contains both larval and recruit samples.

RNA Extraction Protocol

The protocol for this work is documented in my notebook here.

The sample metadata is on GitHub here.

20250613

Today I transferred tissue samples for all larval samples (“A” and “P” sample IDs) from original screw cap cryovials into 1.5 mL safe-lock RNA free Eppendorf tubes.

• Thaw samples on ice (stored in RNA/DNA shield)
• Transfer to new tube labeled with sequencing ID
• Add new RNA/DNA shield to bring total volume to 1000 µL. Use the new RNA/DNA shield to ensure all material is gathered from the original tube.
• Stored at -80°C.

I’ll next complete this process for the remaining recruit samples.