April 2024 Goals

April goals and daily notebook entries.

Goals

Writing and manuscripts

  1. Submit Mcap2021 paper
  2. Draft E5 physiology introduction section
  3. Revise E5 results with updated analysis
  4. Submit Mcap2020

Analysis

  1. DEG analysis of Mcap2023 RNAseq data
  2. QC top off sequencing for Mcap2023 RNAseq data
  3. Measure oyster spat size and enter LCO oyster growth
  4. Analyze LCO oyster qPCR
  5. Collect growth data for LCO
  6. Set up GitHub repos and draw out experimental design for oyster conditioning projects
  7. Re analyze all E5 data using new species identification
  8. Phenoplate and RLC analyses

Sample processing

  1. Larval size photograph analysis for HI 2023
  2. Larval cell density analysis for HI 2023
  3. Larval size analysis for Moorea 2023

Field work and travel planning

  1. Conduct lipidomic and metabolomic research at UTS in Sydney, Australia

Oysters

  1. Arrange field day visit with Taylor Shellfish and Nisbet Oyster Co.
  2. Help write job description for BTC internship
  3. Start next round of seed conditioning

Funding

  1. Send NSF NCE request

Other

  1. Complete Hawaii SAP report for Putnam Lab

Daily Log

April 24

  • Prepared all metabolites for shipment, generated PBQCs
  • Phenoplate analysis and meeting
  • Writing Mcap2023 methods
  • Protein analysis
  • Generating metadata sheets

April 23

  • All protein processing and analysis
  • Writing Mcap2023 methods
  • Protein analysis
  • Phenoplate analysis

April 22

  • Phenoplate analyses
  • Lipid preparation and loading on LCMS

April 20

  • RNA bioinformatics
  • Mcap2020/Mcap2021 revisions
  • Phenoplate analysis
  • RNA DEG analyses

April 19

  • RNA bioinformatics
  • Extraction for UT Sydney samples
  • Phenoplate analysis

April 18

  • Extractions for UT Sydney samples
  • RNAseq bioinformatics
  • Planning meetings and returning emails
  • Mcap 2021 revisions for figures

April 17

  • Extractions for UT Sydney samples
  • RNAseq bioinformatics

April 16

  • Host fraction separations at UT Sydney
  • RNAseq NCBI upload and bioinformatics

April 15

  • Host fraction separations for work at UT Sydney
  • RNAseq NCBI upload

April 14

  • Label tubes and finish trial host fraction separations for work at UT Sydney

April 12

  • Optimizing protocol for host/symbiont separations for lipid and metabolite work at UT Sydney

April 11

  • Lab inductions
  • Practice lipid extractions and preparing reagents for processing
  • Writing protocols for use for experimental M. capitata samples

April 10

  • Arrival in Australia, orientations

April 8

  • Travel to Australia

April 5

  • Shedd cruise meeting
  • Meeting with Flo
  • WSG meetings
  • Prep for Australia
  • Mcap 2021 edits

April 4

  • Roberts Lab meetings
  • Australia prep
  • Oyster ordering and notebooks

April 3

  • Field day at Point Whitney
  • Oyster data management, starting new Git repos
  • CITES permit

April 2

  • Protocols, notebook posts, and data management from last week’s URI trip
  • CITES and Australia trip planning
  • Putnam Lab meeting

April 1

  • Protocols, notebook posts, and data management from last week’s URI trip
  • Australia trip planning
  • WSG meeting
Written on April 19, 2024