This post details metaboloimcs extractions for the 2023 Moorea Symbiotic Exchange project.

Metabolomics Extraction Protocol

See the protocol I am using in my notebook here.

This post details metabolomics extractions for samples taken during the 2023 Symbiotic Exchange project.

20250814

• Prepared bench and hood areas and cleaned all supplies
• Organized/obtained required chemicals
• Labeled tubes
• Located samples

20250819

• Clipped recruit samples and placed into tubes with 1000 µL of LC water
• Move larvae into new labeled tubes (added 200 µL LC water to tubes and then moved to new labeled tube)
• Picked up extraction supplies
• Noticed few larvae in larval tubes, biomass may be low

20250819

• Host-symbiont fraction separations (noticed pellets in larval samples and recruit samples with recruit pellets much larger)
• Completed separations for all samples
• Prepare extraction reagents - 15% ammonium bicarbonate and extraction buffer

20250902

• Extractions in batches of 10: Samples 1-10 (batch 1), 11-20 (batch 2), and 21-30 (batch 3)

20250903

• Extractions for samples 31-40 (batch 4)

20250904

• Final extractions for samples 41-50 (batch 5) and 51-60 (batch 6)
• Boxes prepared for shipment
• Lab areas cleaned
• Note: Sample 47 spilled and is not included in the samples to send (59 total samples)

20250908

• Samples packaged and shipped with dry ice