December goals and daily notebook entries.

Goals

Writing and manuscripts

1. Submit Mcap2020 manuscript to Current Biology
2. Methods for Mcap2023 analysis
3. Submit E5 physiology ms to co-authors and bioRxiv
4. Draft resazurin assay manuscript
5. Methods and results for LCO manuscript

Analysis

1. Analyze LCO oyster qPCR data
2. Phenoplate and RLC analyses for Hawaii 2023
3. Size normalization for 10K seed data
4. Growth analysis for Goose Point seed
5. Growth analysis for Westcott seed with Grace
6. Data analysis for LCO growth
7. TopGO analysis for Hawaii 2023
8. Larval size analysis for Hawaii 2023
9. Larval symbiont density analysis for Hawaii 2023

Field work

1. Goose Point assessment
2. Westcott assessment
3. PolyIC sampling at Point Whitney
4. PolyIC seed transport to UW

Outreach

1. Reva Atea magazine

Presentations

1. Prepare Moorea LTER presentation on E5 work

Daily Log

Dec 30

• E5 phys manuscript
• Reading papers
• Meetings with Emma
• Oyster check on campus
• Cell counts on campus
• Roberts Lab meeting
• Putnam Lab meeting

Dec 26-27

• E5 phys manuscript
• Reading lots of papers

Dec 25

• E5 phys manuscript
• Reading lots of papers
• E5 timeseries molecular WGCNA

Dec 23

• E5 phys manuscript
• Oyster project roadmapping
• Cell counts in the lab
• WGCNA with Kathleen
• PolyIC project design and time line
• Lab meeting

Dec 20

• E5 molecular analysis
• Mcap2020 manuscripts
• Cell counts in the lab
• Water change for oysters and feed oysters

Dec 19

• Mcap2020 manuscript
• Meeting with Yaamini

Dec 18

• Mcap2020 manuscript
• Reefs Unknown meeting
• Reva Atea magazine
• Moorea DNA sequences

Dec 16-17

• Westcott oyster assessment

Dec 13

• Oyster NSF grant
• MCR AIM
• Mcap2020 writing
• E5 writing

Dec 12

• Goose Point temperature data
• MCR AIM
• Meeting with Shayle
• Meeting with Steven - NSF grant
• MCR AIM talk preparation

Dec 11

• Work at Goose Point

Dec 10

• Symbiont cell density preparation and analysis for Hawaii 2023 project
• E5 MCR talk preparations
• Aspen’s defense

Dec 9

• Cleaning oyster tanks at FTR
• Stored and labeled PolyIC sampling tubes from last week
• Lab meeting
• UW SAFS postdoc meet up
• Protocol and started larval symbiont cell density processing

Dec 6

• E5 talk
• E5 molecular meeting
• Resazurin manuscript

Dec 5

• Resazurin manuscript
• E5 analysis
• Meeting with Lucy
• E5 talk

Dec 4

• PolyIC larval sampling at Point Whitney
• Oyster maintenance at Point Whitney

Dec 3

• Working meeting with Yaamini
• Hawaii 2023 top GO coding
• Resazurin manuscript literature searches
• UW Winter Fest

Dec 2

• Putnam lab meeting
• Roberts lab meeting
• Data analysis with Grace
• Review Lucy’s paper
• Moving oysters in FTR to new baskets
• Westcott field logistics planning