WGCNA with eigengene expression plots of ortholog expression for the E5 timeseries molecular project

Today I updated a previously generated WGCNA to include plots that show module eigengene expression against factors of interest.

Overview

Please see my last post for all the details on the WGCNA. Find my previous heatmap with correlations for each module in my past post here. The code can be found here.

Module eigengene expression

As a reminder, here is the heatmap showing module correlations with factors of interest and physiological metrics.

I then generated plots that show module eigengene expression values for each module.

The module eigengene is the first principal component of the expression values of all genes within a module. It represents the dominant shared expression pattern of that module across samples. In other words, the eigengene value for each sample summarizes how “high” or “low” the genes in that module are expressed collectively, allowing us to compare module-level expression across species, timepoints, sites, or physiological states rather than examining individual genes.

Here are some notes on these plots.

They are faceted by WGCNA module (15 total modules)
The metric of interest is on the x-axis
Each panel with a red star shows modules that are significantly correlated with that factor or metric