February 2025 Goals

February goals and daily notebook entries.

Goals

Writing and manuscripts

  1. Revise Mcap2020
  2. Methods for Mcap2023 analysis
  3. Submit E5 physiology ms to co-authors and bioRxiv
  4. Draft resazurin assay 10K seed manuscript
  5. Methods and results for LCO manuscript

Analysis

  1. Phenoplate and RLC analyses for Hawaii 2023
  2. Growth analysis for Goose Point seed (Sept)
  3. Growth analysis for Goose Point seed (Dec)
  4. Data analysis for LCO growth
  5. Larval size analysis for Hawaii 2023
  6. Larval symbiont density analysis for Hawaii 2023
  7. Project Nom Nom PR data analysis
  8. mRNA-lncRNA corelations for deep dive expression
  9. WGCNA for time series expression using multi-omics
  10. Test lipidomic sample analysis
  11. Lucy’s RNAseq bioinformatics

Field work

  1. Plan spring outplant assessment dates

Funding

  1. Submit SeaGrant LOI

Outreach

  1. Reva Atea magazine

Conferences

  1. WSG Shellfish grower meeting

Lab experiments

  1. PolyIC resazurin experiments with UW and undergraduates
  2. PolyIC survival experiments
  3. Cell count processing for HI2023
  4. Purchase plate reader

Daily Log

Feb 27

  • Transport oysters to campus
  • Oyster tanks
  • Clean lab
  • Planning experiments

Feb 26

  • Point Whitney field day

Feb 25

  • COTS project bioinformatic processing
  • Mcap2020 revisions
  • WSG LOI submitted
  • E5 phys paper
  • Budget revisions

Feb 24

  • COTS project bioinformatic processing
  • Mcap2020 revisions
  • WSG LOI
  • Plate reader purchasing

Feb 22

  • COTS project bioinformatic processing
  • Mcap2020 revisions

Feb 21

  • Science hour
  • E5 time series machine learning analyses
  • Mcap2020 revisions

Feb 20

  • Logged into Klone R Studio using these steps
  • Writing meeting for E5 paper with Hollie
  • Roberts Lab meeting
  • Lucy’s bioinformatic data on Unity
  • Mcap2020 revisions

Feb 19

  • Metabolomics paper writing with Yaamini
  • Mcap2020 revisions
  • E5 physiology paper

Feb 18

  • E5 time series test lipid sample analysis
  • E5 physiology paper writing
  • Metabolomics paper writing
  • Methods added to Deep Dive expression paper for WGCNA

Feb 14

  • E5 time series WGCNA analysis
  • E5 molecular meeting
  • Lipid/metabolomics meeting with Yaamini
  • Mcap2020 revisions

Feb 13

  • Survival for Poly IC larvae
  • Lab meeting and photo
  • Lab cleaning
  • Setting up OA equipment

Feb 12

  • Growth and survival data analysis for oyster projects
  • Reviewer reports
  • E5 phys manuscript
  • E5 WGCNA analysis
  • Transfer files from Andromeda to Klone

Feb 10-11

  • WSG meeting

Feb 7

  • Reva Atea magazine
  • Roberts Lab website updates
  • E5 analysis at Science Hour
  • Plate reader quotes

Feb 6

  • Reva Atea magazine
  • RNAseq analysis with Lucy
  • Roberts Lab meeting
  • Roberts Lab website updates
  • Plate reader quotes

Feb 5

  • Meetings with undergrads for project design
  • E5 deep dive expression correlation analyses
  • Plate reader ordering/troubleshooting
  • Oyster tanks and feeding
  • WSG talk meeting
  • E5 physiology manuscript

Feb 4

  • Meetings with undergrads for project design
  • E5 deep dive expression correlation analyses
  • Meeting with Hollie
  • MERLAB meeting

Feb 3

  • Plate reader troubleshooting and ordering supplies
  • Cleaning lab
  • Oyster tank checks
  • Check in’s with undergraduates
  • Meeting with Grace
  • Plan next round of PolyIC experiments
Written on February 27, 2025