February 2025 Goals
February goals and daily notebook entries.
Goals
Writing and manuscripts
- Revise Mcap2020
- Methods for Mcap2023 analysis
- Submit E5 physiology ms to co-authors and bioRxiv
- Draft resazurin assay 10K seed manuscript
- Methods and results for LCO manuscript
Analysis
- Phenoplate and RLC analyses for Hawaii 2023
Growth analysis for Goose Point seed (Sept)- Growth analysis for Goose Point seed (Dec)
- Data analysis for LCO growth
- Larval size analysis for Hawaii 2023
- Larval symbiont density analysis for Hawaii 2023
- Project Nom Nom PR data analysis
mRNA-lncRNA corelations for deep dive expressionWGCNA for time series expression using multi-omicsTest lipidomic sample analysis- Lucy’s RNAseq bioinformatics
Field work
- Plan spring outplant assessment dates
Funding
Submit SeaGrant LOI
Outreach
- Reva Atea magazine
Conferences
WSG Shellfish grower meeting
Lab experiments
- PolyIC resazurin experiments with UW and undergraduates
PolyIC survival experiments- Cell count processing for HI2023
Purchase plate reader
Daily Log
Feb 27
- Transport oysters to campus
- Oyster tanks
- Clean lab
- Planning experiments
Feb 26
- Point Whitney field day
Feb 25
- COTS project bioinformatic processing
- Mcap2020 revisions
- WSG LOI submitted
- E5 phys paper
- Budget revisions
Feb 24
- COTS project bioinformatic processing
- Mcap2020 revisions
- WSG LOI
- Plate reader purchasing
Feb 22
- COTS project bioinformatic processing
- Mcap2020 revisions
Feb 21
- Science hour
- E5 time series machine learning analyses
- Mcap2020 revisions
Feb 20
- Logged into Klone R Studio using these steps
- Writing meeting for E5 paper with Hollie
- Roberts Lab meeting
- Lucy’s bioinformatic data on Unity
- Mcap2020 revisions
Feb 19
- Metabolomics paper writing with Yaamini
- Mcap2020 revisions
- E5 physiology paper
Feb 18
- E5 time series test lipid sample analysis
- E5 physiology paper writing
- Metabolomics paper writing
- Methods added to Deep Dive expression paper for WGCNA
Feb 14
- E5 time series WGCNA analysis
- E5 molecular meeting
- Lipid/metabolomics meeting with Yaamini
- Mcap2020 revisions
Feb 13
- Survival for Poly IC larvae
- Lab meeting and photo
- Lab cleaning
- Setting up OA equipment
Feb 12
- Growth and survival data analysis for oyster projects
- Reviewer reports
- E5 phys manuscript
- E5 WGCNA analysis
- Transfer files from Andromeda to Klone
Feb 10-11
- WSG meeting
Feb 7
- Reva Atea magazine
- Roberts Lab website updates
- E5 analysis at Science Hour
- Plate reader quotes
Feb 6
- Reva Atea magazine
- RNAseq analysis with Lucy
- Roberts Lab meeting
- Roberts Lab website updates
- Plate reader quotes
Feb 5
- Meetings with undergrads for project design
- E5 deep dive expression correlation analyses
- Plate reader ordering/troubleshooting
- Oyster tanks and feeding
- WSG talk meeting
- E5 physiology manuscript
Feb 4
- Meetings with undergrads for project design
- E5 deep dive expression correlation analyses
- Meeting with Hollie
- MERLAB meeting
Feb 3
- Plate reader troubleshooting and ordering supplies
- Cleaning lab
- Oyster tank checks
- Check in’s with undergraduates
- Meeting with Grace
- Plan next round of PolyIC experiments
Written on February 27, 2025