July 2025 Goals

July goals and daily notebook entries.

Goals

Writing and manuscripts

  1. Methods and results for Mcap2023 analysis
  2. Finish full draft of E5 physiology ms
  3. Draft resazurin assay manuscript
  4. Map out LCO data and hardening data for inclusion in manuscript

Analysis

  1. Phenoplate and RLC analyses for Hawaii 2023
  2. Larval size analysis for Hawaii 2023
  3. Larval symbiont density analysis for Hawaii 2023 (UG project)
  4. Project Nom Nom PR data analysis
  5. VIMS resazurin analysis
  6. Revisit respiration scripts for Hawaii 2023 and Moorea 2023 analysis using recent updates with oyster scripts

Field work

  1. Field assessments for Baywater
  2. Outplant PolyIC seed from Point Whitney
  3. Mechanical priming and outplant USDA families at Point Whitney
  4. VIMS field trip
  5. Map out next set of outplants - 10K, PolyIC, USDA

Funding

  1. Write BIO-OCE grant with HP

Lab experiments

  1. Cell count processing for HI2023 (UG project)
  2. Flow cytometer assay testing (UG project)
  3. Resazurin oxygen testing
  4. Metabolomics processing for Moorea2023
  5. RNA extractions for Moorea2023

Daily Log

July 24

  • Meeting with VIMS
  • Meeting with Steven
  • Respiration measurements

July 23

  • Resazurin paper
  • Completed paper review
  • Larval size data

July 22

  • Finished RNA extractions

July 21

  • Oyster oxygen-resazurin correlation experiment resazurin measurements
  • RNA extraction
  • Cleaned lab

July 18

  • Prepped oyster oxygen trials for next week
  • RNA extractions
  • E5 phys manuscript
  • E5 molecular meeting

July 17

  • Manchester oyster maintenance

July 16

  • RNA extractions

July 15

  • Omic paper revisions
  • E5 physiology manuscript writing
  • Larval size images

July 14

  • RNA extractions for Moorea project
  • Omic paper revisions

July 11

  • Larval size analysis
  • E5 physiology paper
  • E5 molecular meeting
  • Omic paper revisions
  • Pacuta larvae paper revisions

July 10

  • Sequim Bay outplants
  • Larval size image analysis

July 9

  • ICB paper revisions
  • Reva Atea magazine
  • Larval size image measurements
  • Oyster project planning
  • Resazurin data and paper

July 8

  • RNA extractions on campus
  • Guava Muse troubleshooting with Aakriti
  • Planning projects with Noah

July 7

  • VIMS trip data analysis
  • E5 physiology manuscript
  • IOS proposal with Hollie
  • Resazurin manuscript
  • Hawaii 2023 data analysis

June 30-July 3

  • VIMS field trip for resazurin work
Written on July 21, 2025