June goals and daily notebook entries.

Goals

Writing and manuscripts

1. Submit Mcap2021 paper
2. Draft E5 physiology introduction section
3. Revise Mcap2020 results

Analysis

1. ~~Mcap2020 functional enrichment~
2. Revise E5 results with updated analysis
3. Analyze LCO oyster qPCR
4. Phenoplate and RLC analyses for Hawaii 2023 project
5. Hawaii 2023 ITS2 QC
6. Hawaii 2023 ITS2 symportal analysis
7. Hawaii 2023 lipidomics analysis
8. Hawaii 2023 metabolomics analysis

Sample processing

1. Larval size photograph analysis for HI 2023
2. Larval cell density analysis for HI 2023

Field planning

1. Budget and approvals for Shedd Cruise trip in August 2024

Oysters

1. Plan June survivorship testing at UW from LCO project
2. Conduct LCO survivorship curve experiment
3. Outplant Westcott oysters
4. Outplant Goose Point oysters

Funding

1. Submit NSF NCE on research.gov

Daily Log

June 27

• Mcap2020 analysis and revising results
• Meeting with Steven
• PolyIC protocols

June 26

• Putnam Lab meeting
• E5 sample selection and freezer inventory prep
• Mcap2020
• Coral data science data preparations

June 25

• Goose Point oyster outplanting

June 24

• Point Whitney, prepping Goose Point outplants

June 21

• Symportal submissions
• Lipid presentation prep
• Mcap2020 analysis
• Lipidomics data analysis
• Oyster data entry and prep for outplanting

June 20

• Oyster data entry
• Oyster notebook entries
• Mcap2020 data
• Meeting with Hollie
• Meeting with Danielle
• Meeting with Shayle
• Submitting full Mcap2021 manuscript metadata

June 19

• Point Whitney field day

June 18

• Campus day
• Meeting with Danielle
• Meeting with Kathleen
• Oyster survival testing
• Mcap2020 analysis

June 17

• Ceabigr revisions
• Mcap2020 analysis
• Oyster data entry

June 14

• Meeting with Jill
• E5 molecular meeting
• Meeting with Dan (eScience)
• Oyster data and notebook posts
• Lipidomics analysis
• Mcap2020 analysis

June 13

• Point Whitney - oyster spawning
• Coral data science preparations
• Data entry and organization for oysters
• GFF troubleshooting with Hollie

June 12

• Point Whitney - organizing, inventory, setting up larval systems

June 11

• Download lipidomic data
• Oyster survival planning
• Writing meeting with Danielle
• AGU postdoc virtual training

June 10

• ceabigr revisions meeting
• Hawaii 2023 ITS2 QC analysis
• Lipid collaboration meeting
• Oyster paper revisions with Eric
• Submit Mcap2021 to PLOS Biology

June 7

• Finished and submitted Mcap2021 paper

June 6

• Oyster hardening paper comments
• Jill’s paper comments
• NSF NCE
• Mcap 2021 writing

June 5

• Putnam Lab meeting
• Hollie meeting for Mcap2021 writing
• eScience postdoc meeting
• Roberts Lab Hackathon
• Oyster planning and data entry

June 4

• Point Whitney field day to prep oysters for Westcott outplant
• Data entry from oyster field day

June 3

• Roberts Lab hackathon
• Prepping Westcott oyster outplants
• Oyster survivorship experiment planning
• E5 sample lists
• Mcap2021 discussion revisions
• Oyster LCO paper planning meeting