June 2025 Goals

June goals and daily notebook entries.

Goals

Writing and manuscripts

  1. Methods and results for Mcap2023 analysis
  2. Finish full draft of E5 physiology ms
  3. Draft resazurin assay manuscript
  4. Map out LCO and hardening manuscript or data to include in another paper

Analysis

  1. Phenoplate and RLC analyses for Hawaii 2023
  2. Larval size analysis for Hawaii 2023 (potential UG projet)
  3. Larval symbiont density analysis for Hawaii 2023 (UG project)
  4. Project Nom Nom PR data analysis
  5. PolyIC, USDA, and 10K resazurin final analysis
  6. Growth analysis for field assessments in spring 2025 (UG projet)

Field work

  1. Field assessments for Baywater
  2. Manchester experiment and outplanting
  3. Outplant PolyIC seed from Point Whitney
  4. Thermal hardening and outplant USDA families at Point Whitney

Funding

  1. Write BIO-OCE grant with HP

Lab experiments

  1. Cell count processing for HI2023 (UG project)
  2. Lab aerial and heat stress trials (UG project)
  3. USDA resazurin trials (UG project)
  4. Flow cytometer assay testing (UG project)
  5. Resazurin oxygen testing
  6. Metabolomics processing for Moorea2023
  7. RNA extractions for Moorea2023
  8. PolyIC lab qPCR experiments (UG project)

Daily Log

June 27

  • VIMS trip planning
  • PW field assessment data management
  • Resazurin paper
  • Review Lucy’s paper

June 26

  • Point Whitney field day and prepping assessments
  • Planning for VIMS trip

June 13

  • Campus day
  • Guava Muse troubleshooting
  • Prepping RNA extractions
  • Oyster tank check
  • Field data analysis

June 12

  • Lab meeting
  • Resazurin data and paper

June 11

  • Manchester logger check
  • Resazurin data and paper

June 10

  • Resazurin paper
  • Oyster growth data

June 9

  • Resazurin paper
  • Oyster growth data

June 5

  • Campus day
  • Cell counts
  • RNA extraction prep
  • Resazurin paper
  • Lab meeting

June 4

  • Campus day
  • Cell counts with Aakriti
  • RNA extraction prep
  • Resazurin paper

June 3

  • Campus day
  • RNA extraction protocols
  • Mapping undergraduate projects
  • Undergraduate work in lab
  • Invoicing and purchasing
  • Resazurin

June 2

  • GitHub and data management for oyster outplants and projects
  • Manchester day to check temperatures
  • Manchester experiment data management
Written on June 26, 2025