March 2024 Goals

March goals and daily notebook entries.

Goals

Writing and manuscripts

  1. Submit Mcap2021 paper
  2. Draft E5 physiology introduction section
  3. Submit Mcap2020

Analysis

  1. Process E5 species ID samples w/ Danielle
  2. ceabigr time series ANOVA and WGCNA results
  3. DEG analysis of Mcap2023 RNAseq data
  4. Measure oyster spat size in images from 3/5 day and 3/19 day
  5. LCO resazurin analysis

Sample processing

  1. Larval size photograph analysis for HI 2023
  2. Larval cell density analyze with Jill for HI 2023
  3. Larval size analysis for Moorea 2023

Field work and travel planning

  1. Finalize CITES for AUS trip
  2. Dates and flights planning for AUS trip - book trip
  3. Week at URI March 24-30

Oysters

  1. Plan next round of WSG/USDA experiments
  2. Attend WSG Shellfish Growers conference March 11-12
  3. Arrange field day visit with Taylor Shellfish and Nisbet Oyster Co.
  4. WSG organizational emails - BTC and field sites

Funding

  1. Draft Sea Grant proposal

Education

  1. Draft coastal erosion lesson plan with Hollie

Daily Log

March 30

  • Notebook posts and protocols

March 29

  • POC and POR species ID for E5
  • ITS2 amplifications
  • CITES and sample preparation
  • Clean and inventory
  • Zymo Switch 3’ library prep

March 28

  • POC and POR species ID for E5
  • ITS2 amplifications
  • CITES list preparation

March 27

  • POC and POR species ID for E5
  • ITS2 sequencing preparation
  • RNA and DNA extractions of Pocillopora larvae with Jill

March 26

  • POC and POR species ID for E5
  • ITS2 DNA dilutions
  • Sample inventory

March 25

  • Australia travel bookings and planning
  • POC and POR species ID for E5
  • Prep ITS2 amplification and sequencing

March 22

  • Australia travel bookings and planning
  • Meeting with Hollie
  • Made list of priorities for work next week at URI
  • E5 Molecular Mechanisms meeting
  • Meeting with shellfish RR Team
  • Meeting with Jill

March 21

  • Ordered new digital caliper
  • Roberts Lab meeting
  • Pubathon meeting
  • Sea Grant proposal
  • Mcap2020 manuscript
  • URI and AUS trip planning

March 20

  • Resazurin preliminary analysis here
  • Putnam Lab meeting
  • Sea Grant proposal
  • Ceabigr manuscript review
  • URI trip planning

March 19

  • Putnam Lab journal club meeting
  • Point Whitney field day
  • Ceabigr manuscript review
  • Sea Grant application

March 18

  • Sea Grant application
  • Meeting with Joe G. - Exploring by the Seat of your Pants
  • Manuscript edits
  • Helped Sarah T. collect anemones

March 15

  • Sea Grant

March 14

  • Roberts Lab meeting
  • Meeting with Steven
  • Pubathon
  • Sea Grant
  • Planning Australia trip

March 13

  • Putnam Lab meeting
  • Sea Grant application

March 12

  • WSG Shellfish Growers Meeting

March 11

  • WSG Shellfish Growers Meeting

March 8

  • E5 molecular meeting and Serena’s defense
  • Sea Grant paperwork
  • ceabigr ASCA analyses

March 7

  • Roberts Lab meeting
  • Testing resazurin liquid respirometry assay with Sam and Steven
  • eScience postdoc seminar
  • eScience meeting with Dan for collaboration
  • Preparing Sea Grant application
  • ceabigr ASCA generating figures and notebook post
  • Submitted eFAST reimbursement requests

March 6

  • Putnam Lab journal club
  • Meeting with Chloe
  • ceabigr writing working time and ASCA analyses
  • Preparing Sea Grant application

March 5

  • Putnam Lab meeting
  • Point Whitney field day for individual growth measurements and planning next round of exposures

March 4

  • GitHub issue for E5 species ID
  • Sea Grant application

March 2-3

  • Worked on SeaGrant proposal over the weekend
  • Revised Coastal Erosion protocol for Hollie with coral reef focus over weekend

March 1

  • Read ~ 30 papers for oyster Sea Grant proposal
  • Sea Grant proposal writing and preparation
Written on March 27, 2024