May goals and daily notebook entries.

Goals

Writing and manuscripts

1. Methods and results for Mcap2023 analysis
2. Finish full draft of E5 physiology ms
3. Draft resazurin assay manuscript with 10K seed, polyIC, and USDA families data
4. Methods and results for LCO manuscript
5. Omic perspectives paper submission

Analysis

1. Phenoplate and RLC analyses for Hawaii 2023
2. Data analysis for LCO growth data
3. Larval size analysis for Hawaii 2023 (potential UG projet)
4. Larval symbiont density analysis for Hawaii 2023
5. Project Nom Nom PR data analysis
6. PolyIC, USDA, and 10K resazurin final analysis
7. Growth analysis for field assessments in spring 2025 (UG projet)
8. E5 metabolomic and energy state analysis

Field work

1. Field assessments for Goose Point
2. Field assessments for Westcott
3. Field assessments for Baywater
4. Field assessments for Point Whitney

Funding

1. Write BIO-OCE grant (draft ready 15 July 2025)

Outreach

1. Reva Atea magazine

Lab experiments

1. Cell count processing for HI2023 (potential UG project)
2. Lab aerial and heat stress trials (UG projet)
3. USDA resazurin trials (UG projet)
4. Flow cytometer assay testing (UG project)
5. Plate reader SOP (UG project)
6. Flow cytometer SOP (UG project)
7. Resazurin field optimization methods and troubleshooting methods
8. Metabolomics processing for Moorea2023
9. RNA extractions for Moorea2023

Daily Log

May 30

• Launch oyster experiment at Manchester

May 29

• Oysters to campus
• Meet with Genevieve
• Meet with Aariki
• Lab meeting
• Prep RNA supplies and protocols
• Resazurin data analysis
• Download temperature logger data
• Resazurin manuscript

May 28

• Sequim Bay field assessment and data management
• Mcap2020 Published!

May 27

• Resazurin data analysis and manuscript
• Meeting with Louis USDA
• Meeting with Ben

May 22

• Lab meeting
• RNA and metabolomic extraction protocols
• Mcap2020 proofs
• Hawaii2023 analyses

May 21

• Goose Point data
• Resazurin data
• RNA and metabolomics protocol drafts
• Meeting with Jackie
• Undergraduate video production

May 20

• Goose Point Field Assessment

May 15-19

• Meeting with Lucy and Hollie
• Meeting with Craig for oyster assays
• Meeting with Kathleen/Steven
• Resazurin data analysis
• URI budget requests

May 15-18

• E5 energetic analyses
• E5 metabolomic analyses

May 14

• Resazurin meeting
• E5 analysis
• Field assessment data processing

May 13

• Westcott Field Assessment
• LCO growth
• Temperature analysis

May 12

• LCO growth analysis
• Travel to Friday Harbor for Westcott assessments

May 9

• Resazurin data analysis
• E5 molecular meeting
• Undergraduate lab work

May 8

• Resazurin data analysis
• Omic paper submission!
• Samples received from URI and organized
• Lab clean up
• Current Biology production forms
• Lab meeting
• Madeline poster presentation

May 7

• Resazurin data analysis and oyster protocol update and management
• Omic paper writing with Yaamini
• Guava ordering
• Started E5 modeling analysis

May 6

• Resazurin with undergraduates
• Order processing and package receipt
• Organizing PolyIC samples in freezer
• Oyster feeding and tank checks
• Met with Madeline regarding undergraduate symposium poster
• Data analysis and GitHub repo for USDA family resazurin trials
• E5 modeler meeting
• Prepping field gear
• Submitted Mcap2020 final revisions - paper accepted

May 5

• Omic paper writing
• Mcap2020 revisions
• Catching up on Issues and emails