Post detailing culture maintenance of embryos of Montipora capitata during June 2021 larval temperature experiment and spawning.

Activities

6/12/2021

• Cultures were stable overnight with minimal mortality at 0800 with developing embryos.

• All conicals and buckets were at ambient temperature under shade cloth. Flow increased to 30mL per 10 sec in conicals. Buckett had bubblers rotating every 3-4 hours.

• Light, PAR, temperature, salinity measurements taken at 1315 recorded on Google Drive data collection sheet.

• Water was changed in all buckets at 1100-1200.

• Cultures look stable and clean!

6/13/2021

• Measured light, pH, temp at 1530

• A. Williams and L. Allen-Waller helped maintain cultures throughout the experiment and monitoring water level in headers/coolers.

• Swimming behavior seen at 2000, ~72 hours post fertilization in samples from previous day’s spawning from H. Putnam larvae

6/14/2021

• Cleaned, drained all conicals and buckets and pooled all together in preparation for baseline sampling and temperature treatments.