Today Steven and I outplanted oysters at Sequim Bay from the PolyIC project.

Overview

In this project we exposed broodstock oysters to Poly(I:C) immune stress and then reared and conducted experiments on the offspring. The seed from these parent groups are now about 8 months old and are ready to be outplanted.

All information for this project can be found on our GitHub repo.

All outplant data is available in the outplant folder here.

Today we outplanted the oysters at Sequim Bay. See my previous post on preparing the oysters for outplants.

Outplanting

Outplanting went well today. We put the oysters in 3/8” mesh brood outplant bags with their identifying tags and temperature loggers. Bags were secured with zip ties to the lines. They will be flipped by the beach crew regularly. Tags were secured on the outside of the bags.

Here is a table with outplant information:

source_tag	outplant_tag	treatment	rearing_tank	number	logger	deploy.date	notes
33	20	Treated	Treated4	100	22023399	20250710	top of health stack, largest group
34	19	Control	Control1	100	22023398	20250710
49	18	Treated	Treated5	100		20250710
39	17	Control	Control2	100		20250710
31	16	Treated	Treated4	100		20250710
60	15	Control	Control1	100		20250710
40	14	Control	Control2	100		20250710
50	13	Treated	Treated5	100		20250710	bottom of stack

Next steps

• Monitor performance over the summer.