Field day at Point Whitney June 12 2024

This post details activities at Point Whitney from 12 June 2024.

Overview

Today we went to Point Whitney to organize and retag all oysters, set up HUDL larval systems, and reset temperature loggers.

I’ve included notes from June 12 and June 13 trips in this post.

Tagging, organizing oysters

Today we organized, moved, and added cattle ear tags to our oysters. A full inventory of tags and current locations can be found on GitHub here.

We moved oysters to the following locations:

  • LCO project: Oysters were moved, but kept on their same rack system into an outdoor upweller. Spat were moved from the silos and into bags. See the list of tags below for more information. Located in the upweller tanks outside of the silos.
  • Effort B: Oysters were kept in an upweller tank with cattle tags added. Located in one silo in an upweller tank.
  • Effort C: Oysters were all moved into one upweller together with cattle tags added. Bags were split between two silos in one upweller.
  • Effort D: Oysters were moved from Heath trays into seed bags in an upweller tank with cattle tags added. Located in one silo in an upweller tank. All oysters from one tray kept in one bag. We have bags for two control trays and two stress trays. See list below for more information.
  • Effort E: Oysters were moved from Heath trays into seed bags in an upweller tank with cattle tags added. Located in one silo in an upweller tank. All oysters from each treatment group were added to one seed bag. We have bags for fresh water treated, fresh water control, temperature treated, and temperature control. See list below for more information. These will be outplanted to Goose Point in a couple weeks.
  • Effort F: Broodstock receievd from USDA ARS kept in adult bags labeled with family with cattle tags added. Adult bags put in the bottom of an upweller tank not in a silo.
  • New seed (USDA ARS 24) were put in the newly opened heath trays. We had one tray per family, labeled by family ID and with a cattle tag included.
  • We tried to tag extras of the Pacific Hybreed families, but some are unknown and we will need to do some DNA work to ID ploidy on these families.

Here are images of all the oysters in upwellers.

cattle_tag effort treatment_type treatment family source tag_description location
8 NA NA NA Unknown Pacific Hybreed Unknown family Outdoor upweller; Point Whitney
13 NA NA NA Unknown Pacific Hybreed Unknown family Outdoor upweller; Point Whitney
31 NA NA NA Unknown Pacific Hybreed Unknown family Outdoor upweller; Point Whitney
17 NA NA NA Unknown Pacific Hybreed Unknown family Outdoor upweller; Point Whitney
2 NA NA NA Unknown Pacific Hybreed Unknown family Outdoor upweller; Point Whitney
10 NA NA NA Toad Pacific Hybreed Toad family Outdoor upweller; Point Whitney
9 NA NA NA Pink Pacific Hybreed Pink family Outdoor upweller; Point Whitney
32 NA NA NA USDA ARS YC24: 52 USDA ARS 2024 POGS Seed Heath tray; Point Whitney
31 NA NA NA USDA ARS YC24: 53 USDA ARS 2024 POGS Seed Heath tray; Point Whitney
27 NA NA NA USDA ARS YC24: 66 USDA ARS 2024 POGS Seed Heath tray; Point Whitney
40 NA NA NA USDA ARS YC24: 55 USDA ARS 2024 POGS Seed Heath tray; Point Whitney
25 NA NA NA USDA ARS YC24: 65 USDA ARS 2024 POGS Seed Heath tray; Point Whitney
1 Effort E Weekly Temperature Control 2023 POGS General Excess USDA ARS Effort E Weekly Temperature Control Outdoor upweller; Point Whitney
20 Effort E Weekly Temperature Treated 2023 POGS General Excess USDA ARS Effort E Weekly Temperature Treated Outdoor upweller; Point Whitney
24 Effort E Weekly Fresh Water Control 2023 POGS General Excess USDA ARS Effort E Weekly Fresh Control Outdoor upweller; Point Whitney
23 Effort E Weekly Fresh Water Treated 2023 POGS General Excess USDA ARS Effort E Weekly Fresh Treated Outdoor upweller; Point Whitney
22 Effort D Weekly Temperature Treated 2023 POGS General Excess USDA ARS Effort D Treated #1 Outdoor upweller; Point Whitney
21 Effort D Weekly Temperature Treated 2023 POGS General Excess USDA ARS Effort D Treated #2 Outdoor upweller; Point Whitney
39 Effort D Weekly Temperature Control 2023 POGS General Excess USDA ARS Effort D Control #1 Outdoor upweller; Point Whitney
38 Effort D Weekly Temperature Control 2023 POGS General Excess USDA ARS Effort D Control #2 Outdoor upweller; Point Whitney
16 Effort B Daily Temperature Treated 2023 POGS General Excess USDA ARS Effort B Treated #1 Outdoor upweller; Point Whitney
15 Effort B Daily Temperature Treated 2023 POGS General Excess USDA ARS Effort B Treated #2 Outdoor upweller; Point Whitney
14 Effort B Daily Temperature Treated 2023 POGS General Excess USDA ARS Effort B Treated #3 Outdoor upweller; Point Whitney
2 Effort B Daily Temperature Control 2023 POGS General Excess USDA ARS Effort B Control #1 Outdoor upweller; Point Whitney
7 Effort B Daily Temperature Control 2023 POGS General Excess USDA ARS Effort B Control #2 Outdoor upweller; Point Whitney
8 Effort B Daily Temperature Control 2023 POGS General Excess USDA ARS Effort B Control #3 Outdoor upweller; Point Whitney
3 Effort C Daily Temperature Control Orange Pacific Hybreed Orange Control #1 Outdoor upweller; Point Whitney
6 Effort C Daily Temperature Treated Orange Pacific Hybreed Orange Treated #2 Outdoor upweller; Point Whitney
5 Effort C Daily Temperature Control Dog Pacific Hybreed Dog Control #1 Outdoor upweller; Point Whitney
4 Effort C Daily Temperature Treated Dog Pacific Hybreed Dog Treated #2 Outdoor upweller; Point Whitney
11 Effort C Daily Temperature Control Blue Pacific Hybreed Blue Control #1 Outdoor upweller; Point Whitney
12 Effort C Daily Temperature Treated Blue Pacific Hybreed Blue Treated #2 Outdoor upweller; Point Whitney
13 Effort C Daily Temperature Control Yellow Pacific Hybreed Yellow Control #1 Outdoor upweller; Point Whitney
19 Effort C Daily Temperature Treated Yellow Pacific Hybreed Yellow Treated #2 Outdoor upweller; Point Whitney
18 Effort C Daily Temperature Control Bird Pacific Hybreed Bird Control #1 Outdoor upweller; Point Whitney
17 Effort C Daily Temperature Treated Bird Pacific Hybreed Bird Control #2 Outdoor upweller; Point Whitney
34 Effort F Broodstock NA 27.014 USDA ARS 27.014 Outdoor upweller; Point Whitney
28 Effort F Broodstock NA 27.025 USDA ARS 27.025 Outdoor upweller; Point Whitney
29 Effort F Broodstock NA 27.052 USDA ARS 27.052 Outdoor upweller; Point Whitney
35 Effort F Broodstock NA 27.009 USDA ARS 27.009 Outdoor upweller; Point Whitney
26 Effort F Broodstock NA 27.039 USDA ARS 27.039 Outdoor upweller; Point Whitney
41 LCO spat LCO Treated 2023 POGS General Excess USDA ARS LCO Spat Treated 1 Outdoor upweller; Point Whitney
42 LCO spat LCO Treated 2023 POGS General Excess USDA ARS LCO Spat Treated 2 Outdoor upweller; Point Whitney
43 LCO spat LCO Control 2023 POGS General Excess USDA ARS LCO Spat Control 1 Outdoor upweller; Point Whitney
44 LCO spat LCO Control 2023 POGS General Excess USDA ARS LCO Spat Control 2 Outdoor upweller; Point Whitney

HUDL systems and tank set up

Steven set up air lines, cleaned the HUDLS, and added heaters to try to get the temperature in the HUDLs up to 25°C. Heaters control temperature in the water bath. I also increased the header tank temperature to 70°F (~21°C) to try to increase the temperature - done at 10:45 am.

Weekly probe and fluorescence measurements

I conducted weekly probe measurements on all tanks and added to GitHub here.

tank date time temp.C pH.nbs sal.psu initials notes
left 6/12/24 15:00 14.2 8.22 29.94 ash indoor left tank
left 6/12/24 15:00 14.1 8.23 30 ash indoor left tank
outdoor_tray 6/12/24 15:00 14.1 7.71 29.64 ash right stack
outdoor_tray 6/12/24 15:00 15.5 7.73 29.55 ash left stack
upweller 6/12/24 15:00 18.9 7.83 29.2 ash effort C
upweller 6/12/24 15:00 18.8 7.83 29.16 ash efforts BED
upweller 6/12/24 15:00 19 7.82 29.05 ash effort F

I took fluorescence measurements on 13 June.

  • Larval indoor tanks = 8, 11, 11 (this is pretty good! We will want to increase to about 15 if we can)
  • Upwellers = 2, 2, 1, 2, 2, 2
  • Heath trays = 3, 4, 2

Weekly Effort E stressors

On 13 June 2024, I conducted weekly Effort E stressors from 11:00-11:30am. I also entered all stressor data from this week and previous weeks on GitHub and shown below.

treatment date time temp.C pH.nbs sal.psu initials notes  
2 fresh 6/13/24 11:20 14.3 8.25 0.276 ash  
3 temperature 6/13/24 11:20 24.3 7.62 30.62 ash  
4 control 6/13/24 11:20 14.7 8.16 29.76 ash  
5 fresh 6/5/24 12:00 15.7 7.97 0.114 ash  
6 temperature 6/5/24 12:00 24.5 7.67 29.75 ash  
7 temperature 5/22/24 12:00 23.5 7.78 30.31 ash  
8 fresh 5/22/24 12:00 14.5 8.31 0.181 ash  
9 temperature 5/16/24 14:45 22 7.62 29.35 ash  
10 fresh 5/16/24 14:45 13.9 8.31 0.057 ash  

Downloading temperature loggers

I downloaded two loggers today. I downloaded SSN 20174086 from heath trays and SSN 20174087 (both pendants) from the left indoor tank. Both of these had a battery error, so data may be incomplete. These were stopped and not relaunced. Data were added to GitHub here.

Downloading Apex data

I downloaded Apex data from January onward using this script. Data were uploaded to GitHub here.

Launching new loggers

We launched new Hobo Tidbit loggers along with all of our groups. Here is the logger metadata.

logger date.launched time.launched date.deployed site model interval tank notes
22023395 20240612 13:45 20240612 Point Whitney Hobo Tidbit MX Temp 5000 15 min Heath tray - 2024 POGS inside tray
22023410 20240612 13:45 20240612 Point Whitney Hobo Tidbit MX Temp 5000 15 min Outdoor upweller - LCO attached to bag
22023398 20240612 13:45 20240612 Point Whitney Hobo Tidbit MX Temp 5000 15 min Outdoor upweller - Effort C attached to bag
22023408 20240612 13:45 20240612 Point Whitney Hobo Tidbit MX Temp 5000 15 min Outdoor upweller - Efforts BED attached to bag
22023406 20240612 13:45 20240612 Point Whitney Hobo Tidbit MX Temp 5000 15 min Outdoor upweller - Effort F attached to bag

Notebook images: June 12

Notebook images: June 13

Written on June 13, 2024