Resazurin sampling for the SORMI project

Resazurin sampling of SORMI families in June 2026

I did not use AI to conduct the work detailed here and did not use AI to write this notebook post.

Overview

Today I helped with the SORMI project family sampling resazurin assays. This post provides an overview of our methods.

Methods

Prior to today, Sam set up a shallow water table with cups glued onto trays for thermal exposures. The water was heated in a header bucket to 35°C, which resulted in a temperature of 33°C in the table.

We measured resazurin in n=9 families (1, 2, 3, 5, 6, 7, 8, 9, and 10) with n=6 replicates per family per round. We ran resazurin in 3 rounds with n=6 blanks per round. Here is the approach we used with this set up.

• Made resazurin stock and working solution as in our standard protocol
• Read fluorescence on plate reader as in standard protocol at 0, 1, 2, and 3 hours of exposure
• Oysters were photographed for size and then added to numbered plastic cups
• Cups were filled with 100 mL resazurin stock solution
• A 200 uL subsample taken from each cup at each time point
• T0 samples were taken immediately after oyster was added to the cup
• Cups placed in recirculating water table with water heated to ~33°C with spot check temperatures recorded every hour
• Analyzed data as in standard protocol
• No mortality observed

Data and output

There was no difference in metabolism between families today.

• Data, code, and figure outputs are found here on GitHub for 20260624 datasets
• Our resazurin standard protocol landing page is here
• SORMI project repo on GitHub