Sequim Bay site growth analysis from 20250531 sampling
Overview
Maddy B. has completed photo analysis for oyster growth at the Sequim site from the 05/31/2026 assessment. This brings analysis of Sequim growth up to date with the most recent assessment.
There are two projects deployed at Sequim Bay: 1) PolyIC cross-generational immune priming projecct and 2) thermal hardening project (Effort A).
In the PolyIC project, parent broodstock were immersed in PolyIC and performance is measured on offspring outplants over the last 2 years.
In the Effort A thermal hardening project, we exposed USDA general excess oysters to two weeks of daily exposure to ~6 h of +10°C conditions and deployed alongside controls.
tl;dr
There was no effect of immune or thermal hardening treatments on field growth.
Data and code
PolyIC project
Data from this assessment are available on GitHub here. The code used to analyse the data is also on GitHub here.
Thermal hardening project (Effort A)
Data from this assessment are available on GitHub here. The code used to analyse the data is also on GitHub here.
Thermal hardening project (Effort A)

No effect of treatment.
Type III Analysis of Variance Table with Satterthwaite's method
Sum Sq Mean Sq NumDF DenDF F value Pr(>F)
treatment 2272 2272 1 5.07 0.6438 0.4583
date 1843507 921753 2 814.96 261.2006 <2e-16 ***
treatment:date 8797 4398 2 814.96 1.2464 0.2881
PolyIC cross-generational project

There was no effect of treatment, only an increase in size with time.
Type III Analysis of Variance Table with Satterthwaite's method
Sum Sq Mean Sq NumDF DenDF F value Pr(>F)
treatment 524 524 1 6.01 0.5342 0.4923
date 67246 33623 2 2501.11 34.2616 2.092e-15 ***
treatment:date 3351 1676 2 2501.11 1.7075 0.1815
Survival was calculated at the level of each bag and was not different between treatments.
