Final assessment and closing 10K experiment at Baywater

Final assessment and closing 10K experiment at Baywater

Overview

On 9/23 we did our final assessment of the Sequim environmental hardening oysters for Effort A and closed out this project! We checked on the PolyIC outplants and left those deployed.

Final assessment

We did the following today to assess and close out the Effort A experiment:

• Collected all Effort A seed bags (7 bags) from the lines and brought them up to the beach
• Removed oysters from each bag and counted mortalities
• Arranged oysters for a photograph to capture number of live animals and for growth analysis
• Tag and scale bar included in each photo
• Subsampled oysters from each bag to total n=~30 oysters per treatment group (2 treatment groups total) into red mesh bags with their own tag labels for survival assays in the lab (see below)
• Bags and equipment were cleaned
• Two temperature loggers were downloaded that were brought in on the bags. There is still one logger remaining with the PolyIC outplants in the field and I added one more to an outplant bag.
• I recorded data and uploaded size image photos.

All data is in our 10K seed GitHub repo.

Temperatures from loggers:

Samples for survival assays

All data is in our 10K seed GitHub repo in the lab survival folder.

Here is a table of the tags collected today from Sequim. I will use this to help Jesse and Maddy prepare datasheets for assays. Each bag has about 30-35 oysters. 30 will be used for collections.

See our methods plan in my previous post here for the survival assays.

tag	experiment	location	treatment
49	Effort A	Sequim	Treated
50	Effort A	Sequim	Control

PolyIC checks

The PolyIC oysters look good! All 8 bags are still deployed.

To do

• Start final photo analysis for growth and survival
• Survival assays in the lab (Jesse & Maddy)