Final assessment and closing 10K experiment at Baywater
Overview
On 9/23 we did our final assessment of the 10K seed at Baywater and closed out this project! We checked on the PolyIC outplants and left those deployed. We also outplanted a trial set of El Nino oysters and SORMI families.
Final assessment
We did the following today to assess and close out the 10K seed experiment:
- Collected all 10K seed bags (24 bags) from the lines and brought them up to the processing facility
- Removed oysters from each bag and counted mortalities
- Arranged oysters for a photograph to capture number of live animals and for growth analysis
- Tag and scale bar included in each photo
- Subsampled oysters from each bag to total n=~30 oysters per treatment group (5 treatment groups total) into red mesh bags with their own tag labels for survival assays in the lab (see below)
- Bags and equipment were cleaned
- Two temperature loggers were downloaded that were brought in on the bags. There is still one logger remaining with the PolyIC outplants in the field. One logger was attached to a mesh bag to follow the oysters to UW.
- I recorded data and uploaded size image photos.
All data is in our 10K seed GitHub repo.
Temperatures from loggers:
Samples for survival assays
All data is in our 10K seed GitHub repo in the lab survival folder.
Here is a table of the tags collected today from Baywater. I will use this to help Jesse and Maddy prepare datasheets for assays. Each bag has about 30-35 oysters. 30 will be used for collections.
See our methods plan in my previous post here for the survival assays.
| tag | experiment | location | treatment |
|---|---|---|---|
| 20 | 10K | Baywater | FW 35C |
| 19 | 10K | Baywater | 35C |
| 18 | 10K | Baywater | PolyIC |
| 17 | 10K | Baywater | FW 35C |
| 16 | 10K | Baywater | Control |
PolyIC checks
The PolyIC oysters grew a lot! We redistributed a couple of over packed bags into other bags from the same treatment group. We also pulled out bag 362 because it was open and empty.
El Nino outplants
We received a shellfish transfer permit (26-1256) to outplant thermal hardened oysters to the field. We did this with the following bags:
| tag | description | number of oysters |
|---|---|---|
| 001 | Family 5 SORMI | 23 |
| 002 | Family 6 SORMI | 24 |
| 003 | El Nino Control | 32 |
| 004 | Family 7 SORMI | 24 |
| 005 | Family 10 SORMI | 23 |
| 006 | El Nino Treated | 32 |
| 007 | Family 2 SORMI | 24 |
| 008 | Family 9 SORMI | 24 |
| 009 | General excess | 50 |
| 010 | General excess | 50 |
| 011 | General excess | 50 |
To do
- Start final photo analysis for growth and survival
- Survival assays in the lab (Jesse & Maddy)